Difference between revisions of "Part:BBa K313002"
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.<br/> | Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.<br/> | ||
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+ | ====Improvements==== | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 08:40, 27 October 2017
flpe DNA recombinase
Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))
We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.
Improvements
•
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 504
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]