Difference between revisions of "Part:BBa K2254999"

Revision as of 08:26, 27 October 2017

SAT toehold swicth

This is an improved version of previous biobrick BBa_K1728019. The design of original biobrick aims to detect spermine N1-acetyltransferase (SAT) mRNA , which is a biomarker of oral cancer. We improved the biobrick by converting the luciferase reporter gene to mRFP reporter gene, which can be visualized by naked eyes to allow on-site detection. We also demonstrated that it can detect SAT transcript.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 105
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 665
Illegal AgeI site found at 777
1000
COMPATIBLE WITH RFC[1000]

Revision as of 04:47, 20 October 2017 (view source) Registry (Talk \| contribs)		Revision as of 08:26, 27 October 2017 (view source) AsaNim (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2254999 short</partinfo>		<partinfo>BBa_K2254999 short</partinfo>

−	This is an improved version of previous biobrick BBa_K1728019. The design of original biobrick aims to detect spermine N1-acetyltransferase (SAT) mRNA , which is a biomarker of oral cancer. We improved the biobrick by converting the luciferase reporter gene to mRFP reporter gene, which can be ~~visible~~ by naked eyes to allow on-site detection. We also demonstrated that it can detect SAT transcript.	+	This is an improved version of previous biobrick BBa_K1728019. The design of original biobrick aims to detect spermine N1-acetyltransferase (SAT) mRNA , which is a biomarker of oral cancer. We improved the biobrick by converting the luciferase reporter gene to mRFP reporter gene, which can be visualized by naked eyes to allow on-site detection. We also demonstrated that it can detect SAT transcript.