Difference between revisions of "Part:BBa K2381002:Design"
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===Source=== | ===Source=== | ||
| − | + | The light-induced protein nMagHigh is made from the VVD protein family derived from the fungus Neurospora crassa. The Rad1 is made from the dCas9 Protein derived from the Streptococcus pyogenes. | |
===References=== | ===References=== | ||
Revision as of 08:22, 27 October 2017
Rad1+ pMagFast
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1371
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2214
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to prevent the fusion protein has steric hindrance, We use a 12aa GS linker to link the light-induced dimerization protein with a part of dCas9, which split by a rational strategy of Cas9. According to the different function and spatial position between α-helix lobe and nuclease, we split dCas9 into two parts. Rad1 includes a-helix lobe, Rad2 includes nuclease lobe.
Source
The light-induced protein nMagHigh is made from the VVD protein family derived from the fungus Neurospora crassa. The Rad1 is made from the dCas9 Protein derived from the Streptococcus pyogenes.