Difference between revisions of "Part:BBa K2382003"
Herlohuang (Talk | contribs) |
Herlohuang (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2382003 short</partinfo> | <partinfo>BBa_K2382003 short</partinfo> | ||
| − | This part originated from pET-29 a (+) Vectors, and it is composed of T7 | + | This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.because the original part has the restriction site”Xba1”in the sequence,and the sequence of this restriction site is” TCTAGA. “ We do the single mutation to convert TC”T”AGA to TC”C”AGA . Thus, it would not effect role to make the composition of our part. |
| − | promoter, Lac operator, and RBS. | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 07:35, 27 October 2017
T7 promoter & Lac operator and RBS from PET-29a
This part originated from pET-29 a (+) Vectors, and it is composed of T7 promoter, Lac operator, and RBS.because the original part has the restriction site”Xba1”in the sequence,and the sequence of this restriction site is” TCTAGA. “ We do the single mutation to convert TC”T”AGA to TC”C”AGA . Thus, it would not effect role to make the composition of our part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]