Difference between revisions of "Part:BBa K1179069"

Latest revision as of 06:53, 27 October 2017

CAG promoter

This part is submitted under the RFC 65 standard.

Encodes the sequence for the CAG promoter.

[http://2017.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt] We improved this part at BBa_K2217013 by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1730
Illegal XbaI site found at 1634
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1730
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1730
Illegal XhoI site found at 12
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1730
Illegal XbaI site found at 1634
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1730
Illegal XbaI site found at 1634
Illegal NgoMIV site found at 1322
Illegal NgoMIV site found at 1479
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 Encodes the sequence for the CAG promoter.
-[http://2017.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt] tried to improve this part at [https://parts.igem.org/Part:BBa_K2217013# BBa_K2217013] by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.
+[http://2017.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt] We improved this part at [https://parts.igem.org/Part:BBa_K2217013# BBa_K2217013] by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.
 <!-- Add more about the biology of this part here