Difference between revisions of "Part:BBa K2302008"
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https://static.igem.org/mediawiki/2017/5/57/NEFU2017%3D%3DRESULT--FIG11.png | https://static.igem.org/mediawiki/2017/5/57/NEFU2017%3D%3DRESULT--FIG11.png | ||
Electrophoresis of PelB-CALB coding fragment (A) | Electrophoresis of PelB-CALB coding fragment (A) | ||
| − | + | Expression cassette of pHisx6-Pelb-CALB (B). | |
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Revision as of 05:54, 27 October 2017
PelB+CALB+His
This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists four basic parts: promoter T7, RBS, pelB and CALB. pelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1023
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 25
Illegal NgoMIV site found at 51
Illegal NgoMIV site found at 744
Illegal NgoMIV site found at 854
Illegal AgeI site found at 546
Illegal AgeI site found at 804 - 1000COMPATIBLE WITH RFC[1000]
result
Electrophoresis of PelB-CALB coding fragment (A) Expression cassette of pHisx6-Pelb-CALB (B).
