Difference between revisions of "Part:BBa K2485012:Design"

Latest revision as of 05:00, 27 October 2017

Proteorhodopsin-cPT-RFP

Assembly Compatibility:

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 ===Design Notes===
-A 5x Gly-Ser linker, BBa_J18922, was used to join proteorhodopsin and cPT.
-===Source===
-The green light absorbing protein rhodopsin was sourced from BBa_K773002 and codons were slightly modified to enhance GC content. It is a proton pump used by marine bacteria for generating proton motive force for ATP generation.
-The cPT Region of IcsA was sourced from BBa_K2485011 with removed start and stop codons, and the last four nucleotides were modified to match the fusion sequence between cPT and GFP in a plasmid provided by Marcia B. Goldberg, MD which was experimentally shown to unipolarly localize GFP.
-FresnoRFP from Atum was used to maximize the distance between the absorption/emission of the fluorescent protein (553/592) and the absorption of the proteorhodopsin (~520).
 ===References===