Difference between revisions of "Part:BBa K2328059"
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+ | ===Usage and Biology=== | ||
+ | We use Linker II and Linker.a to separate smURFP from Acma. The meaningless sequence is 150 bp and has the restriction enzyme cutting sites of XhoI、BglII、NcoI、NheI. | ||
+ | |||
+ | ===Reference=== | ||
+ | [1]Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, et al. (2010) Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model. Vaccine 28: 6684–6691. | ||
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+ | [2]Rodriguez EA,Tran GN , Gross LA, et al. A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein .[J].NATURE METHODS,2016:763-769 |
Latest revision as of 01:29, 27 October 2017
nonsense sequence + Linker II + Linker.a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 165
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 50
Usage and Biology
We use Linker II and Linker.a to separate smURFP from Acma. The meaningless sequence is 150 bp and has the restriction enzyme cutting sites of XhoI、BglII、NcoI、NheI.
Reference
[1]Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, et al. (2010) Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model. Vaccine 28: 6684–6691.
[2]Rodriguez EA,Tran GN , Gross LA, et al. A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein .[J].NATURE METHODS,2016:763-769