Difference between revisions of "Part:BBa K2429012:Design"

(Design Notes)
(Source)
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.
+
Ms2 comes from an RNA bacteriophage
  
 
===References===
 
===References===

Latest revision as of 22:05, 26 October 2017


pENTR Ms2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 294
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene.

Source

Ms2 comes from an RNA bacteriophage

References