Difference between revisions of "Part:BBa K2382001:Design"
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===Source=== | ===Source=== | ||
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https://www.ncbi.nlm.nih.gov/nuccore/118467340/ | https://www.ncbi.nlm.nih.gov/nuccore/118467340/ | ||
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===References=== | ===References=== | ||
Revision as of 20:49, 26 October 2017
MSMEG_5998
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 428
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1 The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)
Source
Mycobacterium smegmatis str. MC2 155 https://www.ncbi.nlm.nih.gov/nuccore/118467340/
References
Lapalikar, G.V., et al., F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the Actinomycetales. PLoS One, 2012. 7(2): p. e30114.
Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.