Difference between revisions of "Part:BBa K2382001:Design"

(→‎Design Notes)
(→‎Design Notes)
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It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression.  
 
It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression.  
 
Otherwise, we remove the stop coden of NC_008596.1
 
Otherwise, we remove the stop coden of NC_008596.1
 +
The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)
  
 
===Source===
 
===Source===

Revision as of 20:00, 26 October 2017


MSMEG_5998


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has not been coden optimized for E. Coli BL21, which however seems to have no effect on protein expression. Otherwise, we remove the stop coden of NC_008596.1 The sequence of this part is from Australian.(While the sequence in BBa_K2382006 is modified by ourselves.)

Source

https://www.ncbi.nlm.nih.gov/nuccore/118467340/


References

Lapalikar, G.V., et al., F420H2-dependent degradation of aflatoxin and other furanocoumarins is widespread throughout the Actinomycetales. PLoS One, 2012. 7(2): p. e30114.

Taylor, M.C., et al., Identification and characterization of two families of F420H2‐dependent reductases from Mycobacteria that catalyse aflatoxin degradation. Molecular microbiology, 2010. 78(3): p. 561-575.