Difference between revisions of "Part:BBa K2206011"

 
 
(5 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2206011 short</partinfo>
 
<partinfo>BBa_K2206011 short</partinfo>
  
−
This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.  
+
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
  
−
This part, in the presence of hsa-miR-27b-3p, is capable of activating BBa_K2206010.
+
The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch. The formation of the duplex occurs through a highly specific binding step which results in single-base mismatch specificity.
 +
 
 +
The trigger RNA sequence for BBa_K2206010 is divided between hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.  
 +
 
 +
For more information, see the section on second series of toehold switches on the design page of CLSB-UK's 2017 wiki - http://2017.igem.org/Team:CLSB-UK/Design
 +
 
 +
{{Template:CLSB-UK 17 Images 27b3p 2}}
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:57, 26 October 2017


Anti-miRNA for hsa-miR-27b-3p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch. The formation of the duplex occurs through a highly specific binding step which results in single-base mismatch specificity.

The trigger RNA sequence for BBa_K2206010 is divided between hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.

For more information, see the section on second series of toehold switches on the design page of CLSB-UK's 2017 wiki - http://2017.igem.org/Team:CLSB-UK/Design

NUPACK Structure Analysis

RBSStart codonTrigger Binding SiteFree energy of secondary structure: -14.08 kcal/molACGUMFE structure at 35.0°Canti-miRNAmiRNAFree energy of secondary structure: -21.70 kcal/molACGUMFE structure at 35.0°C

anti-miRNAmiRNATriggersitebindingRBSStartcodonFree energy of secondary structure: -72.09 kcal/molACGUMFE structure at 35.0°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]