Difference between revisions of "Part:BBa K2368015"
 
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__NOTOC__
 
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
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<partinfo>BBa_K2368015 short</partinfo>
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<p style="text-align: center"><partinfo>BBa_K2368015 short</partinfo></p>
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<p>This part is the overlap of that sweetness receptor T1R2 combines with the GFP tag. </p>
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<p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and GFP tag.</P>
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<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.</p>
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<h1>Design</h1>
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<p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and GFP. Then, we combined the overlap of T1R3 with the GFP by PCR.</p>
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[[File:T-BIT-China-2017parts-27.png|center|500px|默认文字]]
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<p style="text-align: center">Fig.1 The schematic diagram of GFP+T1R2 overlap</p>
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[[File:T-BIT-China-2017parts-28.png|center|500px|默认文字]]
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<p style="text-align: center">Fig. 2 The PCR method of GFP+T1R2 overlap</p>
  
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<P>This part is that sweetness receptor T1R2 combines with the GCP tag. </P>
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<h1>Experiment</h1>
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<P>The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R2 and GFP tag.</p>
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<p>This part is used to detect the expression and the location of the T1R2.</p>
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<P>The result of the part is that the expression of the green fluorescence protein to detect the expression and the location of the T1R2. </p>
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[[File:T-BIT-China-2017parts-29.png|center|300px|默认文字]]
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<p style="text-align: center">Fig.3 Electrophoresis of GFP+T1R2 overlap. </p>
  
  

Latest revision as of 19:11, 26 October 2017


Introduction

T1R2-GFP

This part is the overlap of that sweetness receptor T1R2 combines with the GFP tag.

The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and GFP tag.

The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and GFP. Then, we combined the overlap of T1R3 with the GFP by PCR.

默认文字

Fig.1 The schematic diagram of GFP+T1R2 overlap

默认文字

Fig. 2 The PCR method of GFP+T1R2 overlap

Experiment

This part is used to detect the expression and the location of the T1R2.

默认文字

Fig.3 Electrophoresis of GFP+T1R2 overlap.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]