Difference between revisions of "Part:BBa K2520013"

 
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<partinfo>BBa_K2520013 short</partinfo>
 
<partinfo>BBa_K2520013 short</partinfo>
  
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This device is a complete system for displaying proteins on cells' membrane. The Igk leader (<partinfo>K2520024</partinfo>) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (<partinfo>K2520026</partinfo>) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. HA (<partinfo>K2520030</partinfo>) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of multiple sclerosis (<partinfo>K2520038</partinfo>) and is component related to our specific project.
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The CMV promoter (<partinfo>K1119006</partinfo>) is a constitutive expression promoter in mammalian cells and hGH (<partinfo>K404108</partinfo>) serves as a terminator.
  
 
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Revision as of 18:19, 26 October 2017


CMV-Mono display:MOG 1-hGH

This device is a complete system for displaying proteins on cells' membrane. The Igk leader (BBa_K2520024) is a short signal peptide that prompts the translocation of a protein to the cellular membrane. PDGFR (BBa_K2520026) is a trans-membrane domain that anchors all the components located between the igk leader and the PDGFR itself to the membrane. HA (BBa_K2520030) is a tag that binds to specific antibodies and provide an indirect method for verifying the display of proteins on the membrane. The protein that we chose to express on the membrane is an epitope that is known target of multiple sclerosis (BBa_K2520038) and is component related to our specific project. The CMV promoter (BBa_K1119006) is a constitutive expression promoter in mammalian cells and hGH (BBa_K404108) serves as a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1417