Difference between revisions of "Part:BBa K2217001"
 
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<partinfo>BBa_K2217001 short</partinfo>
 
<partinfo>BBa_K2217001 short</partinfo>
  
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Regulatory circular RNA hsa-circ-0000064 used in Competing endogenous RNA ceRNA circuit, We used this part as a competing endogenuous RNA to regulate Hepatocellular-Carcinoma associated miRNA mir-1825.
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RNA sequence was retrieved from circ-Interactome database Regulatory circular RNA hsa-circ-0000064 used in Competing endogenous RNA ceRNA circuit, We used this part as a competing endogenuous RNA to regulate Hepatocellular-Carcinoma associated miRNA mir-1825.
  
  
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RNAFOLD Model
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==Circular RNA Model==
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Minimum free energy prediction using RNAFOLD generated an optimal secondary structure in dot-bracket notation from a centroid structure of 0.00 kcal/mol minimum free energy Structure
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[[File:Circular RNA (2).png]]  
 
[[File:Circular RNA (2).png]]  
  
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<!-- Add more about the biology of this part here
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to 1.78 kcal/mol minimum free energy Structure
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[[File:Circular RNA.png]]
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More Information about circular design of this part can be found at [http://2017.igem.org/Team:AFCM-Egypt/model# Egypt-AFCM Modeling]
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===Usage and Biology===
 
===Usage and Biology===
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RNA (hsa-circ-0000064) was submitted to the registry after biomarker filtration using bioinformatics databases demonstrated at our biomarker filtration at [http://2017.igem.org/Team:AFCM-Egypt/Design# Egypt-AFCM Design], to be used  as a competing endogenous RNA in a ceRNA network.
  
 
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Latest revision as of 17:17, 26 October 2017


Circular RNA hsa-circ-000064

RNA sequence was retrieved from circ-Interactome database Regulatory circular RNA hsa-circ-0000064 used in Competing endogenous RNA ceRNA circuit, We used this part as a competing endogenuous RNA to regulate Hepatocellular-Carcinoma associated miRNA mir-1825.


Circular RNA Model

Minimum free energy prediction using RNAFOLD generated an optimal secondary structure in dot-bracket notation from a centroid structure of 0.00 kcal/mol minimum free energy Structure

Circular RNA (2).png

to 1.78 kcal/mol minimum free energy Structure

Circular RNA.png

More Information about circular design of this part can be found at [http://2017.igem.org/Team:AFCM-Egypt/model# Egypt-AFCM Modeling]


Usage and Biology

RNA (hsa-circ-0000064) was submitted to the registry after biomarker filtration using bioinformatics databases demonstrated at our biomarker filtration at [http://2017.igem.org/Team:AFCM-Egypt/Design# Egypt-AFCM Design], to be used as a competing endogenous RNA in a ceRNA network.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 128
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]