Difference between revisions of "Part:BBa K1491017"
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===Improvement===
 
===Improvement===
  
−
2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009).
+
2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009). we gave it a new name supernova-lev1([https://parts.igem.org/Part:BBa_K2244010 BBa_K2244010])
  
  

Revision as of 16:10, 26 October 2017

Codon Optimized Supernova

Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.

Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).

389px-LacSuperoxides.jpg
386px-ConstitutiveSuperoxides.jpg


Improvement

2017 SSTi-SZGD Team has improved the this part,The improvement was made by inserting supernova gene into a light-regulated expression system, lightoff (BBa_K2244009). we gave it a new name supernova-lev1(BBa_K2244010)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]