Difference between revisions of "Part:BBa K2269001"
 
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<partinfo>BBa_K2269001 short</partinfo>
 
<partinfo>BBa_K2269001 short</partinfo>
  
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Nopaline synthase terminator of the nopaline synthase gene of Agrobacterium tumefaciens. It's optimized for RFC standards. It is used for gene transfection (Holden, Levine, Scholdberg, Haynes & Jenkins, 2009).
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Middle part for assembly of site-specific recombination based Registers. Composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:26, 26 October 2017


Register Assembly 2/3 (Pr-1a)

Middle part for assembly of site-specific recombination based Registers. Composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]