Difference between revisions of "Part:BBa K2442202"
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This part was intended to be used as regulator construct in our system. The mtlR protein is documented to regulate the activity of the native promoter to Pseudomonas , mtle. Currently it is thought that sugars can induce the dimerisation of the mtlR protein and this complex can in turn activate pmtle. This system can be coupled to GFP to create a reporter construct.
 
This part was intended to be used as regulator construct in our system. The mtlR protein is documented to regulate the activity of the native promoter to Pseudomonas , mtle. Currently it is thought that sugars can induce the dimerisation of the mtlR protein and this complex can in turn activate pmtle. This system can be coupled to GFP to create a reporter construct.
 
We tested the regulatory properties of this part by transforming <i>E.Coli</i> with the reporter plasmid K2442206. <i>iFigure 1</i> illustrates the result of this and indicates the regulatory property of this part works.
 
We tested the regulatory properties of this part by transforming <i>E.Coli</i> with the reporter plasmid K2442206. <i>iFigure 1</i> illustrates the result of this and indicates the regulatory property of this part works.
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<center>{{GlasgowWikiImage|image=T-Glasgow-MTLRresponsegraph.jpg|caption=<b>Figure 1:</b> Incubation time for transformed cells containing different antibiotic resistances}}</center>
  
 
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Revision as of 15:25, 26 October 2017


Ptet + RBS + MtlR

This part contains the ptet promoter (R0040)with a strong ribosome binding site (B0032). These components lie upstream of the protein coding sequence for the mtlR protein native to Pseudomonas fluorescens. This part was intended to be used as regulator construct in our system. The mtlR protein is documented to regulate the activity of the native promoter to Pseudomonas , mtle. Currently it is thought that sugars can induce the dimerisation of the mtlR protein and this complex can in turn activate pmtle. This system can be coupled to GFP to create a reporter construct. We tested the regulatory properties of this part by transforming E.Coli with the reporter plasmid K2442206. iFigure 1 illustrates the result of this and indicates the regulatory property of this part works.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]