Difference between revisions of "Part:BBa K2285019"

Latest revision as of 13:53, 26 October 2017

J23119+Target1+amilCP(RBS+amilCP+Term)

Constitutive promoter J23119 followed by a stem-loop structure Target1, with chromoprotein amilCP as the reporter gene. The original part of amilCP chromoprotein is presented on BBa_K1357009, which is a composite part with B0034 and B0010+B0012 double-terminator.

Usage and Biology

The coding region of amilGFP was originated from iGEM14_Virginia.

Althought Target1 is in the STAR1 system, its structure and function are similar to those of Target3 as a terminator to stop downstream expression of any reporters. Their sequences are different from each other so we can use STAR1 and STAR3 system to control two genetic circuits.

More infomation and details about usage of this part can refer to J23119+Target3+eforRed Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2285019 short</partinfo>
-constitutive promoter J23119 followed by a stem-loop structure Target1, with chromoprotein amilCP as the reporter gene.
+Constitutive promoter J23119 followed by a stem-loop structure Target1, with chromoprotein amilCP as the reporter gene. The original part of amilCP chromoprotein is presented on <partinfo>BBa_K1357009</partinfo>, which is a composite part with B0034 and B0010+B0012 double-terminator.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The coding region of amilGFP was originated from iGEM14_Virginia.
+Althought Target1 is in the STAR1 system, its structure and function are similar to those of Target3 as a terminator to stop downstream expression of any reporters. Their sequences are different from each other so we can use STAR1 and STAR3 system to control two genetic circuits.
+More infomation and details about usage of this part can refer to '''J23119+Target3+eforRed'''
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>