Difference between revisions of "Part:BBa K2350009"
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<partinfo>BBa_K2350009 short</partinfo> | <partinfo>BBa_K2350009 short</partinfo> | ||
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| + | == Part description == | ||
The vector, pPIGBACK, is used to transform into S. elongatus PCC 7942 with the inserted pigment gene in our project. pPIGBACK contains 5’- and 3’-ends of the neutral site II (NSII), replication origin of pBR322, ampicillin resistance gene, and double terminator BBa_B0015. | The vector, pPIGBACK, is used to transform into S. elongatus PCC 7942 with the inserted pigment gene in our project. pPIGBACK contains 5’- and 3’-ends of the neutral site II (NSII), replication origin of pBR322, ampicillin resistance gene, and double terminator BBa_B0015. | ||
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| + | ==Result== | ||
| − | Figure 1 | + | Figure 1 is pPIGBACK-CrtZ transformants electrophoresis result. C1~C20 represents the pPIGBACK-CrtZ transformant clone 1 to clone 20, and M represents 1 kb marker.Transformation efficiency of pPIGBACK-CrtZ is 11.4 transformants per μg DNA, and correctness is 52% (10/19), which is quite efficient because the successful rate of gene double-crossingover homologous recombination is low. See Figure 1. |
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| + | <b>Figure 1 </b> | ||
[[Image:CrtZ Parts.jpg]] | [[Image:CrtZ Parts.jpg]] | ||
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Latest revision as of 13:51, 26 October 2017
Pigment double-crossover homologous gene recombination bacbone (pPIGBACK)
Part description
The vector, pPIGBACK, is used to transform into S. elongatus PCC 7942 with the inserted pigment gene in our project. pPIGBACK contains 5’- and 3’-ends of the neutral site II (NSII), replication origin of pBR322, ampicillin resistance gene, and double terminator BBa_B0015. The strategy we chose to construct the vector is to fuse B0015 and AmpR together first. Secondly, we fused 5’- and 3’-ends of the neutral site II (NSII) with PBR322 replication origin (ORI) together. At last, we ligated two parts together. The aim of constructing this vector is to finish double-crossover homologous gene recombination in S. elongatus PCC 7942. To insert BBa_J04450 with EcoR1 and Pst1 cutting sites, we use site-directed mutagenesis to remove all Pst1 cutting sites in pPIGBACK.
Result
Figure 1 is pPIGBACK-CrtZ transformants electrophoresis result. C1~C20 represents the pPIGBACK-CrtZ transformant clone 1 to clone 20, and M represents 1 kb marker.Transformation efficiency of pPIGBACK-CrtZ is 11.4 transformants per μg DNA, and correctness is 52% (10/19), which is quite efficient because the successful rate of gene double-crossingover homologous recombination is low. See Figure 1.
Figure 1
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 1
Illegal suffix found at 3880 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1
Illegal SpeI site found at 3881
Illegal PstI site found at 3895
Illegal NotI site found at 7
Illegal NotI site found at 1321
Illegal NotI site found at 3888 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1
Illegal BglII site found at 1883
Illegal BamHI site found at 2180
Illegal XhoI site found at 1118 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 1
Illegal suffix found at 3881 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 1
Plasmid lacks a suffix.
Illegal XbaI site found at 16
Illegal SpeI site found at 3881
Illegal PstI site found at 3895
Illegal NgoMIV site found at 2101 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 972
Illegal BsaI site found at 1783