Difference between revisions of "Part:BBa K2520000"
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===Functional Parameters=== | ===Functional Parameters=== | ||
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| + | ===References=== | ||
| + | Cheng, Tian‐Lu, and Steve Roffler. "Membrane‐tethered proteins for basic research, imaging, and therapy." Medicinal research reviews 28.6 (2008): 885-928. | ||
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Revision as of 13:03, 26 October 2017
B7-1 Murine Trans Membrane Domain
The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR.
Figure 1: Membrane-anchored single-chain antibodies. A scFv is composed of the immunoglobulin heavy and light chain variable regions linked together with a flexible peptide (normally 15 amino acids in length). For expression of a membrane-anchored scFv, a signal peptide (SP) directs the nascentchainto the lumen of the endoplasmic reticulum whereas the TM anchors the antibody in the plasma membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]