Difference between revisions of "Part:BBa K2520000"
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The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. | The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. | ||
We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR. | We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR. | ||
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| + | [[File:B7-1.png|800px|thumb|center|Figure 1: Membrane-anchored single-chain antibodies. A scFv is composed of the immunoglobulin heavy and light chain variable regions linked together with aflexible peptide (normally15 amino acids in length).For expression ofa membrane-anchored scFv, asignalpeptide (SP) directsthenascentchaintothe lumenofthe endoplasmic reticulumwhereastheTManchorstheantibody in the plasma membrane. ]] | ||
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Revision as of 12:52, 26 October 2017
B7-1 Murine Trans Membrane Domain
The b7-1 cytoplasmic domain enhances transport from the endoplasmic reticulum and allows accumulation of chimeric proteins on the plasma membrane. It has the same function as PDGFR (the human platelet-derived growth factor receptor), anchoring heterologous proteins on cells' membrane. We used scFv (single chain fragment variable) fused to B7- 1 TM (trans membrane) domain, which according to the literature has increased the expression of scFv on baby hamster kidney cells, approximately 7 fold greater than scFv-PDGFR.
Figure 1: Membrane-anchored single-chain antibodies. A scFv is composed of the immunoglobulin heavy and light chain variable regions linked together with aflexible peptide (normally15 amino acids in length).For expression ofa membrane-anchored scFv, asignalpeptide (SP) directsthenascentchaintothe lumenofthe endoplasmic reticulumwhereastheTManchorstheantibody in the plasma membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]