Difference between revisions of "Part:BBa K2328003"

 
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<partinfo>BBa_K2328003 parameters</partinfo>
 
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===Usage===
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In order to fluoresce, smURFP must be combined with biliverdin (BV). HO-1 is the gene of the precursor of biliverdin. HO-1 can use the materials of the E.coil to produce biliverdin. So we want to construct a plasmid which can both express the smURFP gene and HO-1 gene. Through this construction, we can achieve the co-expression in the E.coil. Both the smURFP and biliverdin are produced by E.coil, so they can connect directly within the E.coil to produce fluorescence under the wavelength of 642 nm without adding BV additonally.
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===Biology===
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One of our methods is co-expression. Because the HO-1 needs to use oxygen to produce BV, it is adoptable in E.coil which is a kind of facultative anaerobic bacteria. And the HO-1 gene is from the Block Library. Both the smURFP and biliverdin are produced by E.coil, so they can connect directly within the E.coil and we can achieve the co-expression in the E.coil.
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===Reference===
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[1] Dong Chen , Jason D Brown , Yukie Kawasaki , Jerry Bommer and Jon Y Takemoto . Scalable production of biliverdin IXα by Escherichia coli. [J].BMC Biotechnology, 2012.

Revision as of 11:14, 26 October 2017


HO-1 II codon-optimized for intestinal bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

In order to fluoresce, smURFP must be combined with biliverdin (BV). HO-1 is the gene of the precursor of biliverdin. HO-1 can use the materials of the E.coil to produce biliverdin. So we want to construct a plasmid which can both express the smURFP gene and HO-1 gene. Through this construction, we can achieve the co-expression in the E.coil. Both the smURFP and biliverdin are produced by E.coil, so they can connect directly within the E.coil to produce fluorescence under the wavelength of 642 nm without adding BV additonally.

Biology

One of our methods is co-expression. Because the HO-1 needs to use oxygen to produce BV, it is adoptable in E.coil which is a kind of facultative anaerobic bacteria. And the HO-1 gene is from the Block Library. Both the smURFP and biliverdin are produced by E.coil, so they can connect directly within the E.coil and we can achieve the co-expression in the E.coil.

Reference

[1] Dong Chen , Jason D Brown , Yukie Kawasaki , Jerry Bommer and Jon Y Takemoto . Scalable production of biliverdin IXα by Escherichia coli. [J].BMC Biotechnology, 2012.