Difference between revisions of "Part:BBa K2368021"
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<h1>Introduction</h1> | <h1>Introduction</h1> | ||
| − | <partinfo>BBa_K2368021 | + | <p style="text-align: center"><partinfo>BBa_K2368021 short</partinfo></p> |
<p> Gα plays an important role in MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. In order to make the sweetness receptor fit the Gα better, we modified the Gα. Then the sweetness receptor can combine with the Gα and send the signal to the downstream.</p> | <p> Gα plays an important role in MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. In order to make the sweetness receptor fit the Gα better, we modified the Gα. Then the sweetness receptor can combine with the Gα and send the signal to the downstream.</p> | ||
| + | [[File:Ga-S-1-HR.png|center|500px|加载失败时候的说明文字]] | ||
| + | <p style="text-align: center"> Fig.1 The structure of Gα in <i>Schizosaccharomyces pombe</i> </p> | ||
<h1>Design</h1> | <h1>Design</h1> | ||
<p> Mutated C-terminus 15bp sequence of the yeast Gα, and replace it with the <i>Schizosaccharomyces pombe</i> Gα subunit. After construct the modified Gα by PCR, and the initial sequence is amplified from <i>Saccharomyces Cerevisiae CEN.PK2-1C</i>.</p> | <p> Mutated C-terminus 15bp sequence of the yeast Gα, and replace it with the <i>Schizosaccharomyces pombe</i> Gα subunit. After construct the modified Gα by PCR, and the initial sequence is amplified from <i>Saccharomyces Cerevisiae CEN.PK2-1C</i>.</p> | ||
| − | + | [[File: GS-1-HR.png|center|500px|加载失败时候的说明文字]] | |
| − | <p> Fig. | + | <p style="text-align: center"> Fig.2 The schematic diagram of Modified Gα-<i>Schizosaccharomyces pombe </i> </p> |
<h1>Experiment</h1> | <h1>Experiment</h1> | ||
| − | <p> The part is used to improve the matching condition of sweetness receptor and the modified Gα. The length of sequence is | + | <p> The part is used to improve the matching condition of sweetness receptor and the modified Gα. The length of sequence is 1419bp.</p> |
| − | + | [[File: 1GS-2-HR.png|center|500px|加载失败时候的说明文字]] | |
| − | <p> Fig. | + | <p style="text-align: center"> Fig.3 Electrophoresis of the positive result of the modified Gα- <i>Schizosaccharomyces pombe</i> </p> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
<partinfo>BBa_K2368021 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2368021 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2368021 arameters</partinfo> | <partinfo>BBa_K2368021 arameters</partinfo> | ||
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Revision as of 10:37, 26 October 2017
Introduction
Modified Gα Schizosaccharomyces pombe
Gα plays an important role in MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. In order to make the sweetness receptor fit the Gα better, we modified the Gα. Then the sweetness receptor can combine with the Gα and send the signal to the downstream.
Fig.1 The structure of Gα in Schizosaccharomyces pombe
Design
Mutated C-terminus 15bp sequence of the yeast Gα, and replace it with the Schizosaccharomyces pombe Gα subunit. After construct the modified Gα by PCR, and the initial sequence is amplified from Saccharomyces Cerevisiae CEN.PK2-1C.
Fig.2 The schematic diagram of Modified Gα-Schizosaccharomyces pombe
Experiment
The part is used to improve the matching condition of sweetness receptor and the modified Gα. The length of sequence is 1419bp.
Fig.3 Electrophoresis of the positive result of the modified Gα- Schizosaccharomyces pombe
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]