Difference between revisions of "Part:BBa K2381018"
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the light-activated split dCas9, which fused the split dCas9 with light-induced dimerization protein, can activated under the irritation of blue light(470nm)
 
the light-activated split dCas9, which fused the split dCas9 with light-induced dimerization protein, can activated under the irritation of blue light(470nm)
−
<partinfo>BBa_K801101</partinfo>
+
<partinfo>BBa_K2381000</partinfo>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:53, 26 October 2017


light-activated rational split dCas9 with rigid linker

the light-activated split dCas9, which fused the split dCas9 with light-induced dimerization protein, can activated under the irritation of blue light(470nm) BBa_K2381000 Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1420
    Illegal NheI site found at 1179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1420
    Illegal BamHI site found at 4495
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1420
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1420
    Illegal AgeI site found at 2357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3155