Difference between revisions of "Part:BBa K1753001"
| Line 4: | Line 4: | ||
This part contains a sequence that codes for RNA polymerase omega subunit. Expressed with other 4 subunits, this subunit can be used to assemble RNA polymerase core enzyme. | This part contains a sequence that codes for RNA polymerase omega subunit. Expressed with other 4 subunits, this subunit can be used to assemble RNA polymerase core enzyme. | ||
| + | |||
| + | It is the smallest subunit of bacterial RNA polymerase (amino acid 91) of E. coli. During assembly of a core enzyme it attaches last and contacts with b’-subunit and only with it. This subunit has been demonstrated to be essential for the correct folding of a b’-subunit, participate in attracting an s-subunit to a core enzyme and be a target for some transcriptional factors regulating the functioning of RNA polymerase (ppGpp). | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | An omega subunit is extracted from cell culture through chromatography in Q sepharose, and eluted with NaCl solution gradient of 0-0.5 M. The major protein fraction comes off at salt concentration of 370-420 mM. | ||
<!-- --> | <!-- --> | ||
| Line 12: | Line 16: | ||
<partinfo>BBa_K1753001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1753001 SequenceAndFeatures</partinfo> | ||
| + | Sequence: | ||
| + | |||
| + | ATGGCACGCGTAACTGTTCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAA | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 06:02, 26 October 2017
RNA polymerase omega subunit
This part contains a sequence that codes for RNA polymerase omega subunit. Expressed with other 4 subunits, this subunit can be used to assemble RNA polymerase core enzyme.
It is the smallest subunit of bacterial RNA polymerase (amino acid 91) of E. coli. During assembly of a core enzyme it attaches last and contacts with b’-subunit and only with it. This subunit has been demonstrated to be essential for the correct folding of a b’-subunit, participate in attracting an s-subunit to a core enzyme and be a target for some transcriptional factors regulating the functioning of RNA polymerase (ppGpp).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Sequence:
ATGGCACGCGTAACTGTTCAGGACGCTGTAGAGAAAATTGGTAACCGTTTTGACCTGGTACTGGTCGCCGCGCGTCGCGCTCGTCAGATGCAGGTAGGCGGAAAGGATCCGCTGGTACCGGAAGAAAACGATAAAACCACTGTAATCGCGCTGCGCGAAATCGAAGAAGGTCTGATCAACAACCAGATCCTCGACGTTCGCGAACGCCAGGAACAGCAAGAGCAGGAAGCCGCTGAATTACAAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAA