Difference between revisions of "Part:BBa K2407100:Design"
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===Design Notes=== | ===Design Notes=== | ||
This sequence is modified for use in yeast or other AT-rich fungi. | This sequence is modified for use in yeast or other AT-rich fungi. | ||
| − | + | yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742 | |
| − | + | It is a composite part to control the expression of HO gene. What's more, it triggers the transformation of their mating type. | |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Hanson SJ, Wolfe KH .An Evolutionary Perspective on Yeast Mating-Type Switching.10.1534/genetics.117.202036. | ||
Latest revision as of 04:20, 26 October 2017
a composite part to control the expression of HO gene
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 866
Illegal EcoRI site found at 1376 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 866
Illegal EcoRI site found at 1376 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 866
Illegal EcoRI site found at 1376
Illegal BglII site found at 902
Illegal BglII site found at 1185
Illegal BglII site found at 1869 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 866
Illegal EcoRI site found at 1376 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 866
Illegal EcoRI site found at 1376
Illegal AgeI site found at 70 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is modified for use in yeast or other AT-rich fungi. yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742 It is a composite part to control the expression of HO gene. What's more, it triggers the transformation of their mating type.
Source
We connect the segments(Gal-HO-CYC1) by T4 DNA Ligase.
References
Hanson SJ, Wolfe KH .An Evolutionary Perspective on Yeast Mating-Type Switching.10.1534/genetics.117.202036.