Difference between revisions of "Part:BBa K2447013"

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<partinfo>BBa_K2447013 short</partinfo>
 
<partinfo>BBa_K2447013 short</partinfo>
  
Tlpa36 proteins is constitutively produced, and depending on the temperature (if below 36 degree), it will form Tlpa36 dimers and capable of repressing the downstream promoter pTlpa36; providing steric hindrance to prevent RNA polymerase to bind and elicit downstream expression.
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TlpA36 protein is constitutively produced, and depending on the temperature it will either form dimers capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form which is unable to repress the promoter.
  
 
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Revision as of 00:55, 26 October 2017


Temperature-sensitive repressible system

TlpA36 protein is constitutively produced, and depending on the temperature it will either form dimers capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form which is unable to repress the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 811
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]