Difference between revisions of "Part:BBa I739203"
Stefan Luzi (Talk | contribs) |
Stefan Luzi (Talk | contribs) |
||
| Line 5: | Line 5: | ||
[[Image:Pck01 Andy.jpg|350px]] | [[Image:Pck01 Andy.jpg|350px]] | ||
| − | In order to make this plasmid compatible with the | + | In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], a MCS harbouring a SpeI site was introduced by PCR. This is the only difference of pCKMCS1 and pCK01.<br> |
[[Image:PCK01-2.jpg]] | [[Image:PCK01-2.jpg]] | ||
| + | ===Purpose=== | ||
| + | <p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains... </p> | ||
Revision as of 20:30, 22 October 2007
No part name specified with partinfo tag.
Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
In order to make this plasmid compatible with the BioBrick Standard assembly process, a MCS harbouring a SpeI site was introduced by PCR. This is the only difference of pCKMCS1 and pCK01.
Purpose
This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains...
References
[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000
Sequence and Features
No part name specified with partinfo tag.