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| − | __NOTOC__
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| − | <h1>Introduction</h1>
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| − | <partinfo>BBa_K2368010 short</partinfo>
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| − | <p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.<p>
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| − | <p>Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and Myc tag. And hope to show the location of T1R3 through immunofluorescence for detecting Myc tag.<p>
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| − | ===Usage and Biology===
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| − | <h2>Sequence and Features</h2>
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| − | <partinfo>BBa_K2368010 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K2368010 parameters</partinfo>
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