Difference between revisions of "Part:BBa K2433001"

Revision as of 18:06, 25 October 2017

ietS

ietS is the gene encoding the toxin in the toxin antitoxin system ietAS native to tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. This part comes from the nopaline type Ti plasmid pTiC58. In combination with the toxin gene, this part has been shown in literature to be important in reducing the transconjugant efficiency for different incoming Ti plasmids as well as contributing to the stability of plasmids harboring this construct. Data base searches have shown that IetA is similar in structure to serine proteases.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 425
Illegal XhoI site found at 2020
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1330
Illegal NgoMIV site found at 2214
Illegal AgeI site found at 2371
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 505
Illegal BsaI.rc site found at 1690
Illegal BsaI.rc site found at 1972

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 <partinfo>BBa_K2433001 short</partinfo>
-etS is the gene encoding the toxin in the toxin antitoxin system ietAS native to tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. This part comes from the nopaline type Ti plasmid pTiC58. In combination with the toxin gene, this part has been shown in literature to be important in reducing the transconjugant efficiency for different incoming Ti plasmids as well as contributing to the stability of plasmids harboring this construct. Data base searches have shown that IetA is similar in structure to serine proteases.
+ietS is the gene encoding the toxin in the toxin antitoxin system ietAS native to tumor-inducing (Ti) plasmids of Agrobacterium tumefaciens. This part comes from the nopaline type Ti plasmid pTiC58. In combination with the toxin gene, this part has been shown in literature to be important in reducing the transconjugant efficiency for different incoming Ti plasmids as well as contributing to the stability of plasmids harboring this construct. Data base searches have shown that IetA is similar in structure to serine proteases.
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