Difference between revisions of "Part:BBa K2235009:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Characterizations of BBa_K2235009=== |
| + | '''Molecular cloning''' | ||
| + | Ligation of sialidase composite insert into pSB1C3 | ||
| + | The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:46, 25 October 2017
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how you used this part and how it worked out.
Characterizations of BBa_K2235009
Molecular cloning Ligation of sialidase composite insert into pSB1C3 The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
User Reviews
UNIQ5b8d1f491f813a11-partinfo-00000000-QINU UNIQ5b8d1f491f813a11-partinfo-00000001-QINU