Difference between revisions of "Part:BBa K2440011"
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<partinfo>BBa_K2440011 short</partinfo> | <partinfo>BBa_K2440011 short</partinfo> | ||
− | It is the target sequence of let-7a, | + | It is the target sequence of let-7a, a modularized DNA part from a set of chemically synthetic oligo DNA library. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | MiRNA locker assembled by using this modularized DNA part, were able to bind let-7a in an Ago2 dependent manner, that is, knockdown of let-7a was achieved by transfecting cells with miRNA locker. | |
===Sequence and Features=== | ===Sequence and Features=== |
Revision as of 16:49, 25 October 2017
let-7a target sequence
It is the target sequence of let-7a, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part, were able to bind let-7a in an Ago2 dependent manner, that is, knockdown of let-7a was achieved by transfecting cells with miRNA locker.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.