Difference between revisions of "Part:BBa K2328027"

 
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<partinfo>BBa_K2328027 parameters</partinfo>
 
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==Results==
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Co-expression with smURFP in E.coli BL21'''
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Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1.
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<p style="text-align: center;">
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    https://static.igem.org/mediawiki/parts/e/e5/Microplate_Reader.png<br>
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'''Table 1.'''  Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.<br>
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</p>
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Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2.
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<p style="text-align: center;">
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    https://static.igem.org/mediawiki/parts/f/f1/Confocal.jpg<br>
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'''Figure 2.'''  The result after induction, the upper one is the control group, and the inferior one is the experimental group.<br>
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</p>
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For other experiments for some intestinal bacteria, we use our codon-optimized gene to express HO-1 in facultative anarobes.
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Revision as of 16:18, 25 October 2017


smURFP I + Linker A + RBS I + HO-1 I


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

Co-expression with smURFP in E.coli BL21

Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1.

Microplate_Reader.png
Table 1. Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.


Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2.

Confocal.jpg
Figure 2. The result after induction, the upper one is the control group, and the inferior one is the experimental group.

For other experiments for some intestinal bacteria, we use our codon-optimized gene to express HO-1 in facultative anarobes.