Difference between revisions of "Part:BBa I742103"

Revision as of 19:06, 22 October 2007

pTG262 Plasmid (Gram negative to Gram positive shuttle vector)

Plasmid contains an origin of replication which is capable of replicating in E. coli and several gram positive organisms including Lactobacillus, Lactococcus and Bacillus. Plasmid has chloramphenicol, neomycin & gentamicin resistance genes. It also has a EcoRI and PstI site with an XbaI site situated in the middle so biobricks can be inserted.

Gene instability may occur with certain inserts (we have not experienced this so far with the biobricks we have inserted).

For more information about pTG262 visit the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Gene_Expression_In_Lactobacillus Edinburgh Yoghurt Wiki]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 463
Illegal XbaI site found at 436
Illegal PstI site found at 424
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 463
Illegal NheI site found at 5313
Illegal PstI site found at 424
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 463
Illegal BglII site found at 2252
Illegal BamHI site found at 442
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 463
Illegal XbaI site found at 436
Illegal PstI site found at 424
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 463
Illegal XbaI site found at 436
Illegal PstI site found at 424
Illegal AgeI site found at 2641
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 2427

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 __NOTOC__
 <partinfo>BBa_I742103 short</partinfo>
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 Gene instability may occur with certain inserts (we have not experienced this so far with the biobricks we have inserted).
+For more information about pTG262 visit the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Gene_Expression_In_Lactobacillus Edinburgh Yoghurt Wiki]
 <!-- Add more about the biology of this part here