Difference between revisions of "Part:BBa K2257007:Design"

 
 
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Formaldehyde sensor sequence  
 
Formaldehyde sensor sequence  
  
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We changed the downstream gene frmAB to RFP and designed the restriction enzyme cutting site to the both ends of this part. Ahead of protein FrmR, we added a flag tag in conveninence of the protein purification and identification.
  
  
 
===Source===
 
===Source===
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+
The origin of the gene sequence is from E.coli K-12.
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Formaldehyde sensor sequence  
+
This protein FrmR acts as natural regulator of protein FrmAB. FrmA and FrmB are two proteins that are participating in the detoxication of formaldehyde in E.coli.
  
  
 
===References===
 
===References===
 +
Katie J Denby et al, The mechanism of a formaldehyde sensing transcriptional regulator, Scientific Reports, 2016(6):38879

Latest revision as of 14:07, 25 October 2017


This part is used for the detection of formaldehyde.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 226
    Illegal XhoI site found at 462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 520


Design Notes

Formaldehyde sensor sequence

We changed the downstream gene frmAB to RFP and designed the restriction enzyme cutting site to the both ends of this part. Ahead of protein FrmR, we added a flag tag in conveninence of the protein purification and identification.


Source

The origin of the gene sequence is from E.coli K-12. This protein FrmR acts as natural regulator of protein FrmAB. FrmA and FrmB are two proteins that are participating in the detoxication of formaldehyde in E.coli.


References

Katie J Denby et al, The mechanism of a formaldehyde sensing transcriptional regulator, Scientific Reports, 2016(6):38879