Difference between revisions of "Part:BBa K2314106"

Revision as of 12:30, 25 October 2017

GH1-1 ( β-glucosidase) coding sequence This part is a gene which encoding a cellobiose transporter(CDT-1).Assimilate cellobiose into intracellular. In our project,we contract an engineered strain to use cellobiose. We all know that S. cerevisiae cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose. Cellobiose is not catabolized by S. cerevisiae and is not accumulated in the cytoplasm.So we express a functional cellodextrin transport system(CDT-1) from N. crassa allow S. cerevisiae to grow with cellobiose as the sole carbon source. CDT-1 is high-affinity cellobiose transporters, with Michaelis constant (Km) values of 4.0T0.3mM, respectively. Cellobiose transport by CDT-1 is inhibited by excess cello-triose, and CDT-1 activity is also inhibited by cellotetraose. What’s more, the combinations of CDT-1 with GH1- 1 constitute fully functional cellodextrin transport systems. We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1115
Illegal BamHI site found at 726
Illegal XhoI site found at 322
Illegal XhoI site found at 985
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 236
Illegal BsaI site found at 376
Illegal BsaI.rc site found at 1030
Illegal SapI site found at 993
Illegal SapI.rc site found at 1377

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2314515 short</partinfo>
+This part is a gene which encoding a cellobiose transporter(CDT-1).Assimilate cellobiose into intracellular.
-This part is a gene Which encoding &#946;-glucosidase.The &#946;-glucosidase capable of hydrolyzing cellobiose into glucose.                                                                               &#946;-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and oligosaccharides, with release of glucose. &#946;-glucosidases catalyze the hydrolysis of soluble cellodextrins to glucose and are a critical component of cellulase systems.In our project,we expressed the GH1-1and CDT-1 in EBY100,the combinations of CDT-1 with GH1- 1 constitute fully functional cellodextrin transport systems.We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.
+In our project,we contract an engineered strain to use cellobiose. We all know that S. cerevisiae cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose.
+Cellobiose is not catabolized by S. cerevisiae and is not accumulated in the cytoplasm.So we express a functional cellodextrin transport system(CDT-1) from N. crassa allow S. cerevisiae to grow with cellobiose as the sole carbon source.                   CDT-1 is high-affinity cellobiose transporters, with Michaelis constant (Km) values of 4.0T0.3mM, respectively. Cellobiose transport by CDT-1 is inhibited by excess cello-triose, and CDT-1 activity is also inhibited by cellotetraose.                                       What’s more, the combinations of CDT-1 with GH1- 1 constitute fully functional cellodextrin transport systems. We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.