Difference between revisions of "Part:BBa K2282007"

 
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<partinfo>BBa_K2282007 short</partinfo>
 
<partinfo>BBa_K2282007 short</partinfo>
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===Usage and Biology===
  
 
AmilCP is a coral chromoprotein which displays a strong blue color. The amilCP expresses a strong color, visible to the naked eye and with a temperature-dependent expression when used with a weak promoter.  
 
AmilCP is a coral chromoprotein which displays a strong blue color. The amilCP expresses a strong color, visible to the naked eye and with a temperature-dependent expression when used with a weak promoter.  
  
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===Source of this part===
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The 5’UTR sequence has been taken from the NCBI genebank on the Cold-shock protein A gene sequence. Mitta et al, 1997 clearly indicated its position so we just had to take the sequence from here. Sequences of the 5’UTR, DSBox and other cold-induced elements are very close to the parts of the iGEM10_Mexico-UNAM-CINVESTAV that can be found here :
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http://partsregistry.org/Part:BBa_K328003
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===Design and considerations===
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This part has no RBS because there is one already located on the 5’UTR, which is the Shine-Dalgarno sequence (Yamanaka et al, 1999) : 5’AAGG 3’
  
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===Usage and Biology===
 
  
 
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Revision as of 12:10, 25 October 2017


BBa_K592009 (AmilCP) supplemented with BBa_K2282002 (CspA 5'UTR)


Usage and Biology

AmilCP is a coral chromoprotein which displays a strong blue color. The amilCP expresses a strong color, visible to the naked eye and with a temperature-dependent expression when used with a weak promoter.

Source of this part

The 5’UTR sequence has been taken from the NCBI genebank on the Cold-shock protein A gene sequence. Mitta et al, 1997 clearly indicated its position so we just had to take the sequence from here. Sequences of the 5’UTR, DSBox and other cold-induced elements are very close to the parts of the iGEM10_Mexico-UNAM-CINVESTAV that can be found here : http://partsregistry.org/Part:BBa_K328003

Design and considerations

This part has no RBS because there is one already located on the 5’UTR, which is the Shine-Dalgarno sequence (Yamanaka et al, 1999) : 5’AAGG 3’


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]