Difference between revisions of "Part:BBa K2505003"

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<span style="margin-left: 25px;">The gene(<i>log1</i>) is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.
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The gene(<i>log1</i>) is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.
  
 
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==Characterization==
 
==Characterization==
  
<span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.
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We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.

Revision as of 10:32, 25 October 2017

IVS-IRES-log1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
    Illegal XhoI site found at 1275
    Illegal XhoI site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The gene(log1) is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.

Characterization

We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.