Difference between revisions of "Part:BBa K2505003"
Line 13: Line 13:
 
<!-- -->
 
<!-- -->
  
−
<span style="margin-left: 25px;">The gene(<i>log1</i>) is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.
+
The gene(<i>log1</i>) is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.
  
 
<html>
 
<html>
Line 22: Line 22:
 
==Characterization==
 
==Characterization==
  
−
<span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.
+
We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.

Revision as of 10:32, 25 October 2017

IVS-IRES-log1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
    Illegal XhoI site found at 1275
    Illegal XhoI site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The gene(log1) is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.

Characterization

We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.