Difference between revisions of "Part:BBa K2217023"
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==Egypt-AFCM Team Improvement== | ==Egypt-AFCM Team Improvement== | ||
− | [http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve YFP function at [https://parts.igem.org/Part:BBa_BBa_K592101# BBa_K592101] regarding its expression by the lac promoter (BBa_K2217017) as a weak constitutive promoter in one composite part to help enhancing YFP expression to characterize non-coding RNA regulatory activity. Part characterization and usage can be found at [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2217023# BBa_K2217023_Experience]. | + | [http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve YFP gel characterization and function at [https://parts.igem.org/Part:BBa_BBa_K592101# BBa_K592101] regarding its expression by the lac promoter (BBa_K2217017) as a weak constitutive promoter in one composite part to help enhancing YFP expression to characterize non-coding RNA regulatory activity. Part characterization and usage can be found at [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2217023# BBa_K2217023_Experience]. |
Revision as of 18:52, 24 October 2017
YFP for ceRNA circuit characterization
Egypt-AFCM Team Improvement
[http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve YFP gel characterization and function at BBa_K592101 regarding its expression by the lac promoter (BBa_K2217017) as a weak constitutive promoter in one composite part to help enhancing YFP expression to characterize non-coding RNA regulatory activity. Part characterization and usage can be found at BBa_K2217023_Experience.
Part BBa_K2217023 is composed of lac operator (BBa_K2217016) and the lac promoter (BBa_K2217017) as a weak constitutive promoter fused to YFP (BBa_K2217014). Finally, it was terminated by Poly_gh termination (BBa_K2217015).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]