Difference between revisions of "Part:BBa K1179062"

Revision as of 17:39, 24 October 2017

LacI

This part is submitted under the RFC 65 standard.

It is the coding sequence for the DNA-binding protein LacI that inhibits the expression of genes being driven by a Lac promoter by binding to the Lac Operon.

Egypt-AFCM Team Improvement

[http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve this part's function [BBa K1179062] regarding inhibition of non-coding RNAs by fusing laci the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site. Part characterization and usage can be found at BBa_K2217027_Experience.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ==Egypt-AFCM Team Improvement==
-[http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve this part's function [BBa K1179062] regarding inhibition of non-coding RNAs by fusing laci  the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site.  Part characterization and usage can be found at  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2217012# BBa_K2217012_Experience].
+[http://2017.igem.org/Design.html# Egypt-AFCM Team] tried to improve this part's function [BBa K1179062] regarding inhibition of non-coding RNAs by fusing laci  the binding site of the miRNA mir-1825 to hsa_circ_0000064 in one composite part to help controlling circular RNA hsa_circ_0000064 transcription at BBa_K2217001 in response to miRNA-1825 binding to this sensor binding site.  Part characterization and usage can be found at  [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2217027# BBa_K2217027_Experience].
 <!-- Add more about the biology of this part here