Difference between revisions of "Part:BBa K2201221"

 
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<partinfo>BBa_K2201221 short</partinfo>
 
<partinfo>BBa_K2201221 short</partinfo>
  
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This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker containing an amber codon is used to show the properties and usages of the ncAA 2-NPA. The streptavidin of this fusion protein can bind to biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. The 2-NPA in the linker induces a cleavage of the peptide backbone when radiated with light at &#654; = 365 nm. After the radiation, the GFP can be removed by simply washing it off the surface, so that the following loss of fluorescence shows the usage of this ncAA.
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This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker containing an amber codon is used to show the properties and usages of the ncAA 2-NPA. The streptavidin of this fusion protein can bind to biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. The 2-NPA in the linker induces a cleavage of the peptide backbone when radiated with light at &#654; = 365 nm.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Important: This part only contains the coding sequence and will not be expressed if transformed. For any further results look up to BBa_K2201321.
  
 
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Revision as of 17:38, 24 October 2017


CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker containing an amber-codon

This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker containing an amber codon is used to show the properties and usages of the ncAA 2-NPA. The streptavidin of this fusion protein can bind to biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. The 2-NPA in the linker induces a cleavage of the peptide backbone when radiated with light at ʎ = 365 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 829
    Illegal AgeI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644