Difference between revisions of "Part:BBa K2314127"

 
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This is an RFP fluorescent device that can be used for fluorescence measurement experiments. The enhancement effect of UTRrpsT on the promoter was demonstrated by comparison test. Any team of interested parties can use it to do the control group on UTRrpsT. The future team can use this as a starting point to design a rich 5'UTR structure.
 
This is an RFP fluorescent device that can be used for fluorescence measurement experiments. The enhancement effect of UTRrpsT on the promoter was demonstrated by comparison test. Any team of interested parties can use it to do the control group on UTRrpsT. The future team can use this as a starting point to design a rich 5'UTR structure.
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This part derives form part BBa_K2314127(https://parts.igem.org/Part:BBa_J23108).
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5'UTR will be transcribed and can be used as a regulatory element to adjust the
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translation process. We chose J23108 and added the 5'UTR sequence (UTRrpsT)
 +
thereafter. We found that 6h fluorescence intensity increased by more than 1.5-
 +
fold. For more information about our new part, please click here:https://parts.igem.org/Part:BBa_K2314127
 +
[[Image:T--OUC-China--improve.illustration.png|500px|thumb|center|UTRrpsT enhances protein expression by structure at the 5’UTR.]]
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[[Image:T--OUC-China-utrrbs.png|500px|thumb|center|The enhancement of UTRrpsT may be related to its secondary structure, as shown by the website (http://rna.tbi.univie.ac.at/).]]
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[[Image:T--OUC-China--FI.png|500px|thumb|center|FI／Abs600： J23106 vs J23106+UTR]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:46, 24 October 2017


RFP Coding Device（Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm)

This is an RFP fluorescent device that can be used for fluorescence measurement experiments. The enhancement effect of UTRrpsT on the promoter was demonstrated by comparison test. Any team of interested parties can use it to do the control group on UTRrpsT. The future team can use this as a starting point to design a rich 5'UTR structure.

This part derives form part BBa_K2314127(https://parts.igem.org/Part:BBa_J23108). 5'UTR will be transcribed and can be used as a regulatory element to adjust the translation process. We chose J23108 and added the 5'UTR sequence (UTRrpsT) thereafter. We found that 6h fluorescence intensity increased by more than 1.5- fold. For more information about our new part, please click here:https://parts.igem.org/Part:BBa_K2314127

UTRrpsT enhances protein expression by structure at the 5’UTR.
The enhancement of UTRrpsT may be related to its secondary structure, as shown by the website (http://rna.tbi.univie.ac.at/).
FI／Abs600： J23106 vs J23106+UTR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 56
    Illegal AgeI site found at 728
    Illegal AgeI site found at 840
  • 1000
    COMPATIBLE WITH RFC[1000]