Difference between revisions of "Part:BBa K2267000"

Latest revision as of 16:24, 24 October 2017

a pH sensitive promoter Pasr

Explained

Asr promoter belongs to asr gene encoding acid-shock RNA. It is a pH-responsive promoter native to E.coli which induces transcription in acidic environment from pH 4.0 ~ 5.0 , and shows low activity at a neutral pH. We chose asr promoter as the base of pH regulation system.
Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 235
Illegal SpeI site found at 2
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 235
Illegal SpeI site found at 2
Illegal NotI site found at 9
Illegal NotI site found at 227
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 235
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 235
Illegal SpeI site found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 235
Illegal SpeI site found at 2
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2267000 short</partinfo>
+<h2>Explained </h2>
-GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.https://static.igem.org/mediawiki/parts/8/8d/GlsA%E6%8F%92%E5%9B%BE.docx
+Asr promoter belongs to asr gene encoding acid-shock RNA. It is a pH-responsive promoter native to E.coli which induces transcription in acidic environment from pH 4.0 ~ 5.0 , and shows low activity at a neutral pH. We chose asr promoter as the base of pH regulation system.https://static.igem.org/mediawiki/2017/8/8c/T--TUST_China--parts_Pasr.png
+<br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===