Difference between revisions of "Part:BBa K2267001"
 
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<partinfo>BBa_K2267001 short</partinfo>
 
<partinfo>BBa_K2267001 short</partinfo>
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<h2>Explained </h2>
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GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.[[File:T--TUST China--parts_GlsA_pcr.png]]
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GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.
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<br>
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Gene sequence: https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=511641&to=512573[[File:T--TUST China--parts GlsA.gif]]
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<h2>Expressed</h2>
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[[File:T--TUST China--parts GlsA pcr.png]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:35, 24 October 2017


A gene encodes glutaminase 1;Catalyze a reaction converting glutamine to glutamate and releasing the

Explained

GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.

Gene sequence: https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=511641&to=512573T--TUST China--parts GlsA.gif

Expressed

T--TUST China--parts GlsA pcr.png
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
    Illegal NotI site found at 9
    Illegal NotI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 967
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]