Difference between revisions of "Part:BBa K2074101"

Latest revision as of 09:32, 24 October 2017

Cry4Aa from Bacillus thuringiensis serovar israelensis strain BRC-LLP29

Bacillus thuringiensis serovar israelensis strain BRC-LLP29 putative cry4A gene, complete cds；GenBank: JQ228565.1

Fig.1 The tertiary structure of Cry4Aa toxin from Bacillus thuringiensis serovar israelensis strain BRC-LLP29

Part BBa_K42415005(https://parts.igem.org/Part:BBa_K2415005) is a standardized and codon-optimized version of this part and it is suitable for protein expression in E.coli.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1638
Illegal EcoRI site found at 1766
Illegal SpeI site found at 612
Illegal SpeI site found at 2502
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1638
Illegal EcoRI site found at 1766
Illegal SpeI site found at 612
Illegal SpeI site found at 2502
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1638
Illegal EcoRI site found at 1766
Illegal BglII site found at 912
Illegal XhoI site found at 980
Illegal XhoI site found at 1799
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1638
Illegal EcoRI site found at 1766
Illegal SpeI site found at 612
Illegal SpeI site found at 2502
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1638
Illegal EcoRI site found at 1766
Illegal SpeI site found at 612
Illegal SpeI site found at 2502
Illegal NgoMIV site found at 2625
1000
COMPATIBLE WITH RFC[1000]

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 https://static.igem.org/mediawiki/igem.org/thumb/9/9f/T--FAFU-CHINA--partsclones.png/800px-T--FAFU-CHINA--partsclones.png
+Part BBa_K42415005(https://parts.igem.org/Part:BBa_K2415005) is a standardized and codon-optimized version of this part and it is suitable for protein expression in E.coli.
 <!-- Add more about the biology of this part here