Difference between revisions of "Part:BBa C0062"

Revision as of 09:28, 24 October 2017

luxR repressor/activator, (no LVA?)

In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr BBa_R0062, and repressing transcription from Pl BBa_R0063.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

Usage and Biology

We improved this part by using it in a part which allows for the constitutive expression of LuxR (BBa_K1893004). This part also includes the pLux promoter, so that genes placed downstream the promoter will be expressed when induced with 3OC6-AHL. We also made a part which allows for the characterisation of LuxR, as we placed GFP downstream the pLux promoter (BBa_K1893005). The part is currently being characterised.

Unspecified.

Sequence and Features

Wenwen Lin(SiCAU-China) |width='60%' valign='top'| Part was sequenced and functional. LuxR was used in our Portable Pathogen Detector. |};