Difference between revisions of "Part:BBa K2491030"

 
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<partinfo>BBa_K2491030 short</partinfo>
 
<partinfo>BBa_K2491030 short</partinfo>
  
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RK2 Broad Host Range Vector with Kanamycin Resistance in PSB1C3 Plasmid
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The goal of this improvement was to replace p15a origin of replication in pSB3K3 (https://parts.igem.org/Part:pSB3K3) with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).
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The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:32, 24 October 2017


RK2 Broad Host Range Vector with Kanamycin Resistance

The goal of this improvement was to replace p15a origin of replication in pSB3K3 (https://parts.igem.org/Part:pSB3K3) with the RK2 ori to generate a shuttle vector as a broad host range vector (i.e. can replicate in E.coli and other microorganisms).

The RK2 origin of replication is a broad-host-range plasmid belonging to the incP incompatibility group that can be maintained in a large number of bacteria. The minimal region for replication and maintenance consists of an origin of replication, oriV, and the plasmid replication initiator protein TrfA protein, that activates oriV. The copy number of RK2 is about 4-7 per cell in E. coli, 3 in Pseudomonas aeruginosa, and 4-7 in Agrobacterium


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1508
    Illegal EcoRI site found at 3141
    Illegal XbaI site found at 3156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1508
    Illegal EcoRI site found at 3141
    Illegal NheI site found at 1384
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 1514
    Illegal NotI site found at 3147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1508
    Illegal EcoRI site found at 3141
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1508
    Illegal suffix found at 2
    Illegal EcoRI site found at 3141
    Illegal XbaI site found at 3156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1508
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3141
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 3156
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1552
    Illegal NgoMIV site found at 2143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.