Difference between revisions of "Part:BBa K2368020"

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__NOTOC__
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<h1>Introduction</h1>
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<partinfo>BBa_K2368020 short</partinfo>
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<p>This part is the overlap of that sweetness receptor T1R3 combines with the BCP tag. </p>
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<p>The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal transduction through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and BCP tag.</P>
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<P>The result of the part is that the expression of the blue cyanidin protein to detect the expression and the location of the T1R3.</p>
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<h1>Design</h1>
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<p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.</p>
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https://static.igem.org/mediawiki/2017/f/f3/BCP3-1.png
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<p>Fig.1 The schematic diagram of BCP+T1R3 overlap</p>
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<h1>Experiment</h1>
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<p>This part is used to detect the expression and the location of the T1R3.</p>
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https://static.igem.org/mediawiki/2017/a/a9/BCP3-2.png
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<p>Fig.2 Electrophoresis of BCP+T1R3 overlap</p>
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===Usage and Biology===
  
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368020 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2368020 parameters</partinfo>
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Revision as of 02:41, 24 October 2017

Introduction

BCP+T1R3 overlap

This part is the overlap of that sweetness receptor T1R3 combines with the BCP tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal transduction through G protein signal pathway. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R3 and BCP tag.

The result of the part is that the expression of the blue cyanidin protein to detect the expression and the location of the T1R3.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R3 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.

BCP3-1.png

Fig.1 The schematic diagram of BCP+T1R3 overlap

Experiment

This part is used to detect the expression and the location of the T1R3.

BCP3-2.png

Fig.2 Electrophoresis of BCP+T1R3 overlap

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]