Difference between revisions of "Part:BBa K2332014"

Line 6: Line 6:
  
  
 
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
[[File:Pblind.png|thumb|center|500px| Figure 1: Blue light inducible expression system. Under blue light, the EL222 DNA binding protein dimerises and bind its binding region within the designed Pblind promoter, overlapping the -35 region of the luxI promoter. This ultimately results in the recruitment of RNAP and transcriptional activation of Intimin'-SpyTag or Intimin'-SpyCatcher. Figure adapted from [https://www.ncbi.nlm.nih.gov/pubmed/27353329 Jayaraman P. et al. (2016)]]]
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 23:20, 23 October 2017


Blue light inducible expression of Intimin'-SpyCatcher (Pblind Intimin'-SpyCatcher)

This biobrick is the fusion of a truncated version of Intimin (159kDa) and SpyCatcher (138 amino acids, 15 kDa) for the cell surface display of SpyCatcher under the control of our blue light inducible promoter (Pblind). To trigger cell adhesion, we decided to make use of the SpyTag and SpyCatcher, two binding partners that form rapid covalent bonds, fused to outer membrane surface proteins. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together post blue light induction.


Usage and Biology

Figure 1: Blue light inducible expression system. Under blue light, the EL222 DNA binding protein dimerises and bind its binding region within the designed Pblind promoter, overlapping the -35 region of the luxI promoter. This ultimately results in the recruitment of RNAP and transcriptional activation of Intimin'-SpyTag or Intimin'-SpyCatcher. Figure adapted from Jayaraman P. et al. (2016)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 1639
  • 1000
    COMPATIBLE WITH RFC[1000]